Open AccessTAP-independent human histocompatibility complex-Cw1 antigen processing of an HIV envelope protein conserved peptide
Author(s) -
Elena Lorente,
Susana Infantes,
Eilon Barnea,
Ilan Beer,
Ruth García,
Fátima Lasala,
Mercedes Jiménez,
Arie Admon,
Daniel López
Publication year - 2011
Publication title -
aids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.195
H-Index - 216
eISSN - 1473-5571
pISSN - 0269-9370
DOI - 10.1097/qad.0b013e328340fe3c
Subject(s) - major histocompatibility complex , human leukocyte antigen , biology , antigen , immune system , peptide , mhc class i , cd8 , virology , antigen presentation , ligand (biochemistry) , transporter associated with antigen processing , immunology , microbiology and biotechnology , biochemistry , t cell , receptor
Individuals with nonfunctional transporters associated with antigen processing (TAP) complexes are not particularly susceptible to viral infections or neoplasms. Therefore, their immune system must be reasonably efficient, and the present, though reduced, cytolytic CD8 αβ T subpopulation specific for TAP-independent antigens may be sufficient to establish an immune defense protecting against viral infections in these individuals. The objective of the present study was to identify TAP-independent ligands from HIV gp160 protein. An analysis and comparison of complex human histocompatibility complex (HLA)-bound peptide pools isolated from large quantities of healthy or HIV gp160-expressing human cells was performed using mass spectrometry and bioinformatics tools. A conserved TAP-independent HLA peptide ligand endogenously processed and presented in infected human cells was identified. This ligand originates from the envelope protein bound to the HLA-Cw1 class I molecule with high affinity. It was concluded that HLA class I peptides derived from a large fraction of the N-terminal HIV envelope protein could be presented even in the absence of the TAP complex.