Open AccessA defucosylated bispecific multivalent molecule exhibits broad HIV-1-neutralizing activity and enhanced antibody-dependent cellular cytotoxicity against reactivated HIV-1 latently infected cells
Author(s) -
Desheng Kong,
Yan Wang,
Ping Ji,
Wei Li,
Tianlei Ying,
Jinghe Huang,
Chen Wang,
Yanling Wu,
Yanping Wang,
Weizao Chen,
Yanling Hao,
Kui Hong,
Yiming Shao,
Dimiter S. Dimitrov,
Shibo Jiang,
Liying Ma
Publication year - 2018
Publication title -
aids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.195
H-Index - 216
eISSN - 1473-5571
pISSN - 0269-9370
DOI - 10.1097/qad.0000000000001869
Subject(s) - antibody dependent cell mediated cytotoxicity , virology , antibody , cytotoxicity , cell culture , flow cytometry , neutralizing antibody , biology , cytotoxic t cell , peripheral blood mononuclear cell , microbiology and biotechnology , gp41 , immunology , in vitro , virus , monoclonal antibody , epitope , biochemistry , genetics
Current treatments cannot completely eradicate HIV-1 owing to the presence of latently infected cells, which harbor transcriptionally silent HIV-1. However, defucosylated antibodies can readily kill latently infected cells after their activation to express envelope glycoprotein (Env) through antibody-dependent cellular cytotoxicity (ADCC). We herein aimed to test a defucosylated bispecific multivalent molecule consisting of domain-antibody and single-domain CD4, LSEVh-LS-F, for its HIV-1 neutralizing activity and ADCC against the reactivated latently infected cells, compared with the nondefucosylated molecule LSEVh-LS.