Open AccessTissue Thickness Interferes With the Estimation of the Immunohistochemical Intensity: Introduction of a Control System for Managing Tissue Thickness
Author(s) -
Shinobu Masuda,
Ryôhei Suzuki,
Yuriko Kitano,
Haruishimaki,
Hiroko Kobayashi,
Yoko Nakanishi,
Hayato Yokoi
Publication year - 2020
Publication title -
applied immunohistochemistry and molecular morphology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.868
H-Index - 60
eISSN - 1541-2016
pISSN - 1533-4058
DOI - 10.1097/pai.0000000000000859
Subject(s) - immunohistochemistry , intensity (physics) , context (archaeology) , staining , biomedical engineering , pathology , materials science , computer science , medicine , optics , biology , physics , paleontology
The conversion of immunohistochemical (IHC) results from 3-dimensional tissue to a 2-dimensional visual image without considering tissue thickness poses a considerable risk of misleading IHC intensities. The present study aimed to clarify whether tissue thickness interferes with the estimation of IHC staining intensity and to introduce a control system to manage it.