Open AccessGram‐negative Microbiota Blooms in Premature Twins Discordant for Parenteral Nutrition‐associated Cholestasis
Author(s) -
Hourigan Suchitra K.,
Moutinho Thomas J.,
Berenz Andrew,
Papin Jason,
Guha Pallabi,
Bangiolo Lois,
Oliphant Sandra,
Provenzano Marina,
Baveja Raj,
Baker Robin,
Vilboux Thierry,
Levy Shira,
Deopujari Varsha,
Nataro James P.,
Niederhuber John E.,
Moore Sean R.
Publication year - 2020
Publication title -
journal of pediatric gastroenterology and nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.206
H-Index - 131
eISSN - 1536-4801
pISSN - 0277-2116
DOI - 10.1097/mpg.0000000000002617
Subject(s) - medicine , cholestasis , necrotizing enterocolitis , confounding , microbiome , neonatal intensive care unit , gut flora , neonatal cholestasis , gastroenterology , immunology , pediatrics , biology , liver transplantation , bioinformatics , biliary atresia , transplantation
Parenteral nutrition‐associated cholestasis (PNAC) causes serious morbidity in the neonatal intensive care unit. Infection with gut‐associated bacteria is associated with cholestasis, but the role of intestinal microbiota in PNAC is poorly understood. We examined the composition of stool microbiota from premature twins discordant for PNAC as a strategy to reduce confounding from variables associated with both microbiota and cholestasis. Eighty‐four serial stool samples were included from 4 twin sets discordant for PNAC. Random Forests was utilized to determine genera most discriminatory in classifying samples from infants with and without PNAC. In infants with PNAC, we detected a significant increase in the relative abundance of Klebsiella, Veillonella, Enterobacter , and Enterococcus ( P < 0.05). Bray‐Curtis dissimilarities in infants with PNAC were significantly different ( P < 0.05) from infants without PNAC. Our findings warrant further exploration in larger cohorts and experimental models of PNAC to determine if a microbiota signature predicts PNAC, as a basis for future interventions to mitigate liver injury.