Open AccessMixed Lipid, Fish Oil, and Soybean Oil Parenteral Lipids Impact Cholestasis, Hepatic Phytosterol, and Lipid Composition
Author(s) -
Isaac Daniela Migliarese,
Alzaben Abeer S.,
Mazurak Vera C.,
Yap Jason,
Wizzard Pamela R.,
Nation Patrick N.,
Zhao YuanYuan,
Curtis Jonathan M.,
Sergi Consolato,
Wales Paul W.,
Mager Diana R.,
Turner Justine M.
Publication year - 2019
Publication title -
journal of pediatric gastroenterology and nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.206
H-Index - 131
eISSN - 1536-4801
pISSN - 0277-2116
DOI - 10.1097/mpg.0000000000002313
Subject(s) - stigmasterol , phytosterol , campesterol , cholestasis , medicine , soybean oil , triglyceride , fish oil , linoleic acid , endocrinology , cholesterol , chemistry , food science , biochemistry , fatty acid , biology , chromatography , sterol , fish <actinopterygii> , fishery
ABSTRACT Objectives: In parenteral nutrition‐dependent infants and children, intestinal failure (IF)‐associated liver disease (IFALD) remains an important problem. A comparative study was undertaken of parenteral mixed lipid (ML), ω‐3 predominant fish oil (FO), and ω‐6 predominant soybean oil (SO) emulsions in regards to hepatic phytosterol, neutral lipid, fatty acid (FA) content, and the relationship to cholestasis in piglets. Methods: Neonatal piglets received parenteral nutrition, varying in lipid dose (5 or 10 g· kg −1 · day −1 ) and formulation: SO5 (n = 5), SO10 (n = 5), FO5 (n = 5), and ML10 (n = 5). On day 14, liver chemistry, bile flow, histology and neutral lipid staining were assessed. Hepatic triglyceride FA content was determined using thin layer and gas chromatography, and phytosterol content was assessed using gas chromatography‐mass spectrometry. Results: SO groups had higher prevalence of biochemical cholestasis ( P < 0.04) and lower bile flow ( P < 0.0001). Hepatic campesterol, stigmasterol, and β‐sitosterol were highest in SO10 ( P < 0.0001). Hepatic FA ( P < 0.03) and ω‐6/ω‐3 FA ratio ( P < 0.0001) were higher in the SO groups. Neutral lipid accumulation ( P = 0.3) and liver histology ( P = 0.16) were not different between groups. Univariate predictors of bile flow were: campesterol ( r = −0.77, P = 0.001), β‐sitosterol ( r = −0.74, P = 0.002), stigmasterol ( r = −0.74, P = 0.002), ω‐6 FA ( r = −0.72, P = 0.002), and ω‐3 FA ( r = 0.59, P = 0.02). Only campesterol independently predicted bile flow. Conclusions: ML and FO lipid emulsions reduce cholestasis in association with lowered hepatic phytosterol and lipid content. Lower hepatic phytosterol and ω‐6 FA content, and higher ω‐3 FA content are hepatoprotective. Multivariate analysis suggests reduced phytosterol accumulation may best explain the hepatoprotective effect of fish oil‐containing lipids.