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Cigarette Smoke Activates NFκB and Induces Muc5b Expression in Mouse Middle Ear Cells
Author(s) -
Preciado Diego,
Lin Jezhin,
Wuertz Beverly,
Rose Mary
Publication year - 2008
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1097/mlg.0b013e31815aedc7
Subject(s) - reporter gene , transcription factor , microbiology and biotechnology , gene expression , nfkb1 , chemistry , reverse transcription polymerase chain reaction , biology , gene , biochemistry
Objectives: Cigarette smoke exposure is a significant risk factor in the development of otitis media (OM). Nuclear factor kappa B (NF‐κB) is a ubiquitous transcription factor known to mediate cigarette smoke effects on gene regulation in multiple cell types. The MUC5B mucin gene contains several putative NF‐κB sites in its promoter and is the predominant mucin expressed in human OM. We hypothesized that in vitro stimulation of a recently developed model system, murine middle ear epithelial cells (MEEC), with cigarette smoke condensate (CSC) activates NF‐κB and subsequently induces Muc5b gene expression. Methods: Luciferase reporter assays, electromobility shift assays (EMSA), and quantitative microplate transcription factor assays (TFA) were performed to evaluate NF‐κB activation with CSC in immortalized murine MEEC (mMEEC). Reverse transcriptase polymerase chain reaction (RT‐PCR) assays and quantitative real time RT‐PCR were performed to determine whether time course CSC stimulation upregulates Muc5b mRNA levels in differentiated mMEEC. Luciferase reporter assays were performed to determine whether CSC activates the Muc5b promoter. Results: Reporter assays, EMSA, and TFA demonstrated three‐ to five‐fold dose‐dependent activation of NF‐κB with CSC in mMEEC. CSC stimulation likewise increased Muc5b mRNA abundance and induced reporter activity 1.8‐ to 4.8‐fold in plasmids containing −556 and −255 base pairs upstream of the Muc5b transcriptional start site in mMEEC. Conclusions: CSC activates NF‐κB in immortalized MEEC. Furthermore, this activation correlates with CSC‐induced Muc5b promoter activation and gene expression. Taken together, these results hint that much as in lung cells, the activation of mucins by cigarette smoke is mediated in part by NF‐κB.