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Quantitative Analysis of Syndecan‐1 Expression in Dysplasia and Squamous Cell Carcinoma of the Oral Cavity
Author(s) -
Jackson Lana L.,
Wade Zane,
Hessler Richard B.,
Abdelsayed Rafik,
Rogers Jeremy B.,
Gourin Christine G.
Publication year - 2007
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1097/mlg.0b013e318033c810
Subject(s) - dysplasia , syndecan 1 , grading (engineering) , pathology , immunohistochemistry , epithelial dysplasia , carcinoma , medicine , staining , stage (stratigraphy) , cancer , carcinoma in situ , cell , biology , ecology , paleontology , genetics
: Decreased expression of syndecan‐1 has been reported in dysplasia and squamous cell carcinoma (SCCA) of the oral cavity and appears to correlate with decreasing histological differentiation and poor clinical outcome. Assays of syndecan‐1 expression to date have utilized manual microscopic analysis with qualitative grading of immunohistochemical staining intensity, which may introduce observer bias. We evaluated syndecan‐1 expression in dysplasia and squamous cell carcinoma (SCCA) of the oral cavity, using a novel automated cellular imaging system that incorporates both staining intensity as well as the percentage of positively stained cells to yield a quantitative value for syndecan‐1 expression. Materials and Methods : We performed a quantitative immunohistochemical analysis of syndecan‐1 expression using an automated cellular image analysis system. We analyzed specimens from cases of mild dysplasia (N = 55), moderate dysplasia (N = 38), severe dysplasia (N = 25), carcinoma in situ (CIS) (N = 43), and SCCA of the oral cavity (N = 45), using normal mucosal epithelium (N = 21) as a positive control. The SCCA specimens were further subdivided by degree of differentiation. We retrospectively reviewed patient charts to identify tumor stage at diagnosis, recurrence, and disease‐specific survival. Results : Syndecan‐1 expression was significantly greater in normal controls than in specimens of mild, moderate, or severe dysplasia, CIS, or invasive SCCA ( P < .05). Syndecan‐1 expression did not differ significantly among specimens of mild, moderate, or severe dysplasia, CIS or SCCA. There was no significant difference in syndecan‐1 expression between specimens from patients with no evidence of disease at 3 years follow‐up and patients with local, regional, or distant recurrence. Conclusions : Syndecan‐1 expression does not appear to be useful as a marker of differentiation or as a prognostic indicator in dysplasia and SCCA of the oral cavity. The search for a suitable and reliable marker of biological aggressiveness is ongoing.