Open AccessMicroarray analysis of differentially expressed microRNAs in myelodysplastic syndromes
Author(s) -
Chengyao Wan,
Jing Wen,
Ying Huang,
Hongying Li,
Wei Wu,
Qiongni Xie,
Xiaolin Liang,
Zhongyuan Tang,
Weihua Zhao,
Cheng Pan,
Zhenfang Liu
Publication year - 2020
Publication title -
medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.59
H-Index - 148
eISSN - 1536-5964
pISSN - 0025-7974
DOI - 10.1097/md.0000000000020904
Subject(s) - microrna , pathogenesis , microarray analysis techniques , myelodysplastic syndromes , bone marrow , microarray , medicine , haematopoiesis , dna microarray , real time polymerase chain reaction , gene expression profiling , downregulation and upregulation , cancer research , bioinformatics , computational biology , immunology , biology , gene expression , gene , genetics , stem cell
Background: Our study aimed to analyze differential microRNA expression between myelodysplastic syndromes (MDS) and normal bone marrow, and to identify novel microRNAs relevant to MDS pathogenesis. Methods: MiRNA microarray analysis was used to profile microRNA expression levels in MDS and normal bone marrow. Quantitative real-time polymerase chain reaction was employed to verify differentially expressed microRNAs. Results: MiRNA microarray analysis showed 96 significantly upregulated (eg, miR-146a-5p, miR-151a-3p, miR-125b-5p) and 198 significantly downregulated (eg, miR-181a-2-3p, miR-124-3p, miR-550a-3p) microRNAs in MDS compared with normal bone marrow. The quantitative real-time polymerase chain reaction confirmed the microarray analysis: expression of six microRNAs (miR-155-5p, miR-146a-5p, miR-151a-3p, miR-221-3p, miR-125b-5p, and miR-10a-5p) was significantly higher in MDS, while 3 microRNAs (miR-181a-2-3p, miR-124-3p, and miR-550a-3p) were significantly downregulated in MDS. Bioinformatics analysis demonstrated that differentially expressed microRNAs might participate in MDS pathogenesis by regulating hematopoiesis, leukocyte migration, leukocyte apoptotic process, and hematopoietic cell lineage. Conclusions: Our study indicates that differentially expressed microRNAs might play a key role in MDS pathogenesis by regulating potential relevant functional and signaling pathways. Targeting these microRNAs may provide new treatment modalities for MDS.