Open AccessHuman papilloma virus identification in ocular surface squamous neoplasia by p16 immunohistochemistry and DNA chip test
Author(s) -
Tina Shrestha,
Won Pil Choi,
Ga-Eon Kim,
JaeHo Yang,
Kyung Chul Yoon
Publication year - 2019
Publication title -
medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.59
H-Index - 148
eISSN - 1536-5964
pISSN - 0025-7974
DOI - 10.1097/md.0000000000013944
Subject(s) - medicine , immunohistochemistry , human papilloma virus , pathology , serotype , hpv infection , cancer , dermatology , virology , cervical cancer
The aim of this study was to identify the association between human papilloma virus (HPV) infection and ocular surface squamous neoplasia (OSSN) using p 16 immunohistochemistry (IHC) and deoxyribonucleic acid (DNA) chip test. Thirty-eight patients who underwent surgical excision of OSSN were retrospectively studied using tissue samples. The IHC was performed to assess the expression of p 16 and DNA chip test was used to detect 24 HPV serotypes. Among the 38 OSSN samples, 32 cases (84.2%) were histopathologically categorized as pre-invasive type and 6 cases (15.8%) as invasive type. The IHC for p 16 showed strong positivity in 12 cases (31.6%), whereas it was negative in 26 cases (68.4%). On the other hand, only one case (2.6%) of invasive OSSN was positive for the HPV16 serotype, as assessed by DNA chip test. In OSSN, p 16 expression was positive in approximately 1/3rd of the cases, whereas the majority of the 24 HPV serotypes were negative for p 16. Our findings suggest that only a weak association exists between HPV infection and OSSN.