Open AccessEpigenetic differences of chronic hepatitis B in different TCM syndromes
Author(s) -
Li Ma,
Xiuli Zheng,
Yu Yang,
Jian Wang,
Yingming Xu,
Baojia Wang
Publication year - 2018
Publication title -
medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.59
H-Index - 148
eISSN - 1536-5964
pISSN - 0025-7974
DOI - 10.1097/md.0000000000012452
Subject(s) - medicine , epigenetics , dna methylation , traditional chinese medicine , disease , bioinformatics , gene , genetics , pathology , biology , alternative medicine , gene expression
Chronic hepatitis B is a serious disease causing serious harm to the human health. Chinese medicine has its unique advantages in the clinical prevention and treatment, while the syndrome of Chinese medicine lacks the understanding at the micro level. There are some theoretical commonalities between the epigenetics and traditional Chinese medicine (TCM) syndromes. The biological basis of chronic hepatitis B (CHB) syndrome differentiation from the perspective of epigenetics is of great significance to diagnose and prevent the diseases. Methods: This protocol is a case-control, noninterventional, observational clinical study. Patients with CHB for spleen-stomach damp heat and liver depression and spleen deficiency, with 12 each and 11 healthy volunteers were recruited. Peripheral venous blood was collected from the participants. DNA methylated transferase, genomic DNA methylated spectrum, methylated DNA binding protein MeCP2, chronic infection of hepatitis B virus with methylated related proteins, and miRNA target genes were analyzed. Objectives: From the perspective of DNA methylation epigenetics, “DNA methylation–miRNA–Target gene” is the main line, which further reveals the essence of TCM syndrome. To improve the level of TCM clinical syndrome differentiation and the clinical efficacy of TCM, especially in the study of TCM syndromes of CHB, discovering its underlying biological signature is necessary. Trial registration: Clinical Trials Registration: ChiCTR1800017365, registered 26 July 2018.