Open AccessPrimed Left Ventricle Heart Perfusion Creates Physiological Aortic Pressure in Porcine Hearts
Author(s) -
Liming Xin,
Weiran Yao,
Yan Peng,
Pengzhou Lu,
Roberto Vanin Pinto Ribeiro,
Bin Wei,
B. Gellner,
Craig A. Simmons,
Jean W. Zu,
Yu Sun,
Mitesh Badiwala
Publication year - 2020
Publication title -
asaio journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.961
H-Index - 66
eISSN - 1538-943X
pISSN - 1058-2916
DOI - 10.1097/mat.0000000000000947
Subject(s) - ventricle , perfusion , cardiology , medicine , afterload , ventricular pressure , pulse pressure , diastole , contractility , isovolumetric contraction , blood pressure , coronary perfusion pressure , hemodynamics , circulatory system , anesthesia , cardiopulmonary resuscitation , resuscitation
This article presents a primed left ventricle heart perfusion method to generate physiologic aortic pressure (AoP) and perform functional assessment. Isolated hearts of male Yorkshire pigs were used to study the hemodynamic behaviors of AoPs generated in the primed left ventricle heart perfusion (n = 6) and conventional (zero-loaded left ventricle) Langendorff perfusion (n = 6). The measurement results show that left ventricular pressure generated in the primed left ventricle heart perfusion is a determinant of physiologic AoP (i.e. systolic and diastolic pressures within physiologic range). The aortic pulse pressure (systolic pressure = 124.5 ± 1.7 mm Hg, diastolic pressure = 87.8 ± 0.9 mm Hg, aortic pulse pressure = 36.7 ± 2.6 mm Hg) from the primed left ventricle heart perfusion represents close match with the in vivo physiologic data. The volume in the left ventricle remains constant throughout the primed left ventricle heart perfusion, which allows us to perform isovolumetric left ventricular pressure measurement in ex vivo heart perfusion (EVHP). Left ventricular contractility measurements (maximum and minimum rates of left ventricular pressure change) were derived for cardiac assessment. In summary, the proposed primed left ventricle heart perfusion method is able to create physiologic AoP and enables left ventricular functional assessment in EVHP in porcine hearts.