Open AccessEpidermal growth factor receptor mutations in non–small cell lung cancer undetected by high-sensitivity allele-specific real-time polymerase chain reaction–based assays
Author(s) -
Ching-Hui Shen,
HsiangLing Ho,
YiChen Yeh,
ChaoHua Chiu,
TehYing Chou
Publication year - 2020
Publication title -
journal of the chinese medical association
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.535
H-Index - 42
eISSN - 1728-7731
pISSN - 1726-4901
DOI - 10.1097/jcma.0000000000000277
Subject(s) - sanger sequencing , epidermal growth factor receptor , medicine , lung cancer , exon , polymerase chain reaction , cold pcr , mutation , t790m , kras , cancer research , allele , microbiology and biotechnology , cancer , gene , oncology , point mutation , biology , genetics , gefitinib , colorectal cancer
Identifying epidermal growth factor receptor (EGFR) mutation status is critical for planning lung cancer treatment. Sanger sequencing detects both known and novel mutations but shows poor sensitivity. High-sensitivity allele-specific real-time polymerase chain reaction (ASRP)-based assays offer quick and reliable results, but may overlook uncommon mutations. We aimed to define the rate at which high-sensitivity ASRP-based assays missed uncommon EGFR mutations.