Open AccessPhenotypic and microRNA characterization of the neglected CD24+ cell population in MCF-7 breast cancer 3-dimensional spheroid culture
Author(s) -
Lily Boo,
Swee Keong Yeap,
Norlaily Mohd Ali,
Wan Yong Ho,
Huỳnh Kỳ,
Dilan Amila Satharasinghe,
Woan Charn Liew,
Sheau Wei Tan,
MongLien Wang,
Soon Keng Cheong,
Hooi Tin Ong
Publication year - 2020
Publication title -
journal of the chinese medical association
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.535
H-Index - 42
eISSN - 1728-7731
pISSN - 1726-4901
DOI - 10.1097/jcma.0000000000000226
Subject(s) - homeobox protein nanog , cd44 , cancer stem cell , cd24 , spheroid , cancer research , population , mcf 7 , wnt signaling pathway , microbiology and biotechnology , stem cell , cancer cell , biology , cell culture , cell , medicine , cancer , signal transduction , genetics , induced pluripotent stem cell , embryonic stem cell , human breast , environmental health , gene
In vitro 3-dimensional (3D) spheroid culture has been widely used as model to enrich CD44CD24 cancer stem cells (CSC) with high aldehyde dehydrogenase 1 (ALDH1) activity. Although CD24 subpopulation was known to be present in 3D spheroids and may influence cancer drug therapies, its characteristics and CSC properties were not well defined.