Open AccessAbsence of Gastrointestinal Pathogens in Ileum Tissue Resected for Necrotizing Enterocolitis
Author(s) -
T. Ullrich,
Yi Tang,
Hernán Correa,
Steven Garzon,
Akhil Maheshwari,
Melissa L. Hill,
Pranathi Matta,
Mohan K. Krishnan,
Jörn-Hendrik Weitkamp
Publication year - 2012
Publication title -
the pediatric infectious disease journal/the pediatric infectious disease journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 140
eISSN - 1532-0987
pISSN - 0891-3668
DOI - 10.1097/inf.0b013e318242534a
Subject(s) - necrotizing enterocolitis , ileum , enterocolitis , enteritis , medicine , polymerase chain reaction , ileitis , biology , virology , pathology , gastroenterology , disease , crohn's disease , biochemistry , gene
Necrotizing enterocolitis (NEC) is one of the most common gastrointestinal emergencies in premature infants and has been linked with viral antigens in as much as 40% of cases in single-center cohorts. We examined 28 tissue sections from surgically resected ileum from 27 preterm infants with NEC from 2 separate institutions for 15 common bacterial, viral, and parasitic gastrointestinal pathogens using multiplex reverse transcriptase polymerase chain reaction amplification and suspension array detection methods. We did not detect infectious enteritis pathogens in any of the NEC tissues and conclude that gastrointestinal pathogens are a rare cause of NEC.