Open AccessDetection of Mycobacterium Tuberculosis DNA in Buccal Swab Samples from Children in Lima, Peru
Author(s) -
Juan Flores,
Roger Calderón,
Annelies W. Mesman,
Martín Soto,
Julia Coit,
Juan Aliaga,
Milagros Mendoza,
Segundo R. León,
Kelika A. Konda,
Francisco M. Mestanza,
Carlos Javier Mendoza,
Leonid Lecca,
Megan Murray,
Rebecca C. Holmberg,
Nira R. Pollock,
Molly F Franke
Publication year - 2020
Publication title -
the pediatric infectious disease journal/the pediatric infectious disease journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 140
eISSN - 1532-0987
pISSN - 0891-3668
DOI - 10.1097/inf.0000000000002828
Subject(s) - buccal swab , medicine , mycobacterium tuberculosis , tuberculosis , polymerase chain reaction , confidence interval , mycobacterium tuberculosis complex , buccal administration , microbiology and biotechnology , pathology , biology , dentistry , genetics , gene
We examined Mycobacterium tuberculosis DNA detection from buccal swab samples collected from children in Lima, Peru. DNA was extracted and amplified via real-time polymerase chain reaction. Sensitivity was 21% (95% confidence interval [CI]: 7%-42%) in 24 culture-confirmed tuberculosis cases and 4.6% (95% CI: 1%-13%) in 65 clinically diagnosed unconfirmed cases. Sensitivity was highest for smear-positive tuberculosis. Specificity was 99% in the 199 controls (95% CI: 96%-100%).