Open AccessComparison of Saliva Pcr Assay Versus Rapid Culture for Detection of Congenital Cytomegalovirus Infection
Author(s) -
Swetha Pinninti,
Shan A. Ross,
Masako Shimamura,
Zdeněk Novák,
April Palmer,
Amina Ahmed,
Robert W. Tolan,
David I. Bernstein,
Marian G. Michaels,
Pablo J. Sánchez,
Karen B. Fowler,
Suresh B. Boppana
Publication year - 2015
Publication title -
the pediatric infectious disease journal/the pediatric infectious disease journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 140
eISSN - 1532-0987
pISSN - 0891-3668
DOI - 10.1097/inf.0000000000000609
Subject(s) - saliva , cytomegalovirus , medicine , betaherpesvirinae , cytomegalovirus infections , virology , herpesviridae , polymerase chain reaction , human cytomegalovirus , real time polymerase chain reaction , viral disease , immunology , virus , biology , gene , genetics
As part of the CMV and Hearing Multicenter Screening (CHIMES) study, 72,239 newborns were screened for cytomegalovirus by rapid culture and real-time PCR of saliva samples. Of the 266 infants with congenital cytomegalovirus infection, discordance between rapid culture and PCR was observed in 14 children, and 13 were identified only by PCR, demonstrating the superiority of the PCR assay.