Open AccessGenetic Characterization of Streptococcus pyogenes emm89 Strains Isolated in Japan From 2011 to 2019
Author(s) -
Yujiro Hirose,
M. Yamaguchi,
Norihiko Takemoto,
Tohru MiyoshiAkiyama,
Tomoko Sumitomo,
Masao Nakata,
Tadayoshi Ikebe,
Tomoki Hanada,
Takahiro Yamaguchi,
Ryuji Kawahara,
Rumi Okuno,
Hitoshi Otsuka,
Yuko Matsumoto,
Yuji Terashima,
Yu Kazawa,
Noriko Nakanishi,
Kaoru Uchida,
Yumi Akiyama,
Kaori Iwabuchi,
Chikara Nakagawa,
Kazunari Yamamoto,
Victor Nizet,
Shigetada Kawabata
Publication year - 2020
Publication title -
infectious microbes and diseases
Language(s) - English
Resource type - Journals
eISSN - 2096-7241
pISSN - 2641-5917
DOI - 10.1097/im9.0000000000000038
Subject(s) - streptococcus pyogenes , virulence , biology , clade , microbiology and biotechnology , streptococcus , gene , whole genome sequencing , genome , virology , genetics , bacteria , phylogenetics , staphylococcus aureus
Abstract Invasive infection caused by Streptococcus pyogenes emm 89 strains has been increasing in several countries linked to a recently emergent clade of emm 89 strains, designated clade 3. In Japan, the features of emm 89 S. pyogenes strains, such as clade classification, remains unknown. In this study, we collected emm 89 strains isolated from both streptococcal toxic shock syndrome (STSS) (89 STSS isolates) and noninvasive infections (72 non-STSS isolates) in Japan from 2011 to 2019, and conducted whole-genome sequencing and comparative analysis, which resulted in classification of a large majority into clade 3 regardless of disease severity. In addition, invasive disease-associated factors were found among emm 89 strains, including mutations of control of virulence sensor, and absence of the hylP1 gene encoding hyaluronidase. These findings provide new insights into genetic features of emm 89 strains.