Open AccessA Fixed-Depth Microneedle Enhances Reproducibility and Safety for Corneal Gene Therapy
Author(s) -
Brian C. Gilger,
Elizabeth Crabtree,
Liujiang Song,
Telmo Llanga,
Megan Cullen,
Allison Blanchard,
John L. Salmon,
Sarvar Patel,
Vladimir G. Zarnitsyn,
Matthew Hirsch
Publication year - 2019
Publication title -
cornea
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.274
H-Index - 117
eISSN - 1536-4798
pISSN - 0277-3740
DOI - 10.1097/ico.0000000000002182
Subject(s) - reproducibility , ophthalmology , medicine , biomedical engineering , optometry , chemistry , chromatography
Drug delivery directly to the corneal stroma currently relies on microscopic injections that demonstrate low reproducibility and clinician-dependent variability. With use of biological drugs such as adeno-associated viral (AAV) vectors, precise and consistent drug deposition is critical to reduce concerns related to off-target transduction and the host's immune response to the viral capsid and/or transgene-derived product. Therefore, a precise corneal injection (PCI) microneedle was designed to allow accurate depth-specific injections into the corneal stroma in a macroscopic setting.