Open AccessFibrinography: A Multiwavelength Light‐Scattering Assay of Fibrin Structure
Author(s) -
Dassi Carhel,
Seyve Landry,
García Xabel,
Bigo Emmanuelle,
Marlu Raphaël,
Caton François,
Polack Benoît
Publication year - 2019
Publication title -
hemasphere
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 11
ISSN - 2572-9241
DOI - 10.1097/hs9.0000000000000166
Subject(s) - fibrin , fibrinogen , plasma , coagulation , cascade , chemistry , thrombin , biophysics , chromatography , immunology , biochemistry , platelet , biology , physics , medicine , quantum mechanics
We have previously developed a fibrin structural assay dedicated to purified fibrinogen‐thrombin system. Here, we extend the pertinence of this test, called Fibrinography, to tissue factor‐triggered plasma coagulation. We show that Fibrinography determines quantitatively the structure of fibrin fibers in plasma with an excellent reproducibility. We compare this assay with the commonly used single wavelength turbidity method, showing that the latter is not a proper structural assay, but determines essentially the fibrinogen content in plasma. In addition, we also show, in model plasmas, that Fibrinography is able to discriminate normal and hypocoagulant plasmas, and even between hypercoagulant plasmas. Therefore, Fibrinography, by measuring the final step of the coagulation cascade, may be used to evaluate patients’ plasma in hypo‐ or hypercoagulant diseases.