Open AccessDo Patient-derived Spheroid Culture Models Have Relevance in Chondrosarcoma Research?
Author(s) -
Rong Ma,
Jonathan B. Mandell,
Fang Lu,
Tanya Heim,
Karen Schoedel,
Anette Duensing,
Rebecca Watters,
Kurt R. Weiss
Publication year - 2020
Publication title -
clinical orthopaedics and related research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.178
H-Index - 204
eISSN - 1528-1132
pISSN - 0009-921X
DOI - 10.1097/corr.0000000000001317
Subject(s) - chondrosarcoma , spheroid , medicine , pathology , cell culture , h&e stain , cancer research , biology , immunohistochemistry , genetics
In high-grade chondrosarcoma, 5-year survival is lower than 50%. Therefore, it is important that preclinical models that mimic the disease with the greatest possible fidelity are used to potentially develop new treatments. Accumulating evidence suggests that two-dimensional (2-D) cell culture may not accurately represent the tumor's biology. It has been demonstrated in other cancers that three-dimensional (3-D) cancer cell spheroids may recapitulate tumor biology and response to treatment with greater fidelity than traditional 2-D techniques. To our knowledge, the formation of patient-derived chondrosarcoma spheroids has not been described.