Open AccessDDIT4 mediates the proliferation-promotive effect of IL-34 in human monocytic leukemia cells
Author(s) -
Xiaoqian Lv,
Yuting Hu,
Lina Wang,
Dongyue Zhang,
Hao Wang,
Yibo Dai,
Xiaoxu Cui,
Zheng Ge
Publication year - 2021
Publication title -
blood science
Language(s) - English
Resource type - Journals
ISSN - 2543-6368
DOI - 10.1097/bs9.0000000000000069
Subject(s) - gene knockdown , downregulation and upregulation , leukemia , apoptosis , cytokine , cancer research , cell growth , microbiology and biotechnology , biology , chemistry , immunology , gene , biochemistry
Interleukin 34 (IL-34) is a cytokine that shares the receptor with colony-stimulating factor 1 (CSF-1). IL-34 is involved in a broad range of pathologic processes including cancer. We previously demonstrated that IL-34 promoted the proliferation and colony formation of human acute monocytic leukemia (AMoL) cells. However, the mechanism has not been elucidated. Here, by analyzing the gene profiles of Molm13 and THP1 cells overexpressing IL-34 (Molm13-IL-34 and THP1-IL-34) , upregulation of the DNA damage-inducible transcript 4 (DDIT4) was detected in both series. Knockdown of DDIT4 effectively inhibited the proliferation, promoted apoptosis and colony formation in Molm13-IL-34 and THP1-IL-34 cells. Our results suggest that DDIT4 mediates the proliferation-promotive effect of IL-34 whereas does not mediate the promotive effect of IL-34 on colony formation in AMoL cells.