Open AccessTwo-step protocol for regeneration of immunocompetent T cells from mouse pluripotent stem cells
Author(s) -
Tongjie Wang,
Chen Lv,
Fang Hu,
Lijuan Liu,
Jinyong Wang
Publication year - 2020
Publication title -
blood science
Language(s) - English
Resource type - Journals
ISSN - 2543-6368
DOI - 10.1097/bs9.0000000000000049
Subject(s) - transplantation , microbiology and biotechnology , induced pluripotent stem cell , biology , stem cell , in vivo , in vitro , regeneration (biology) , progenitor cell , haematopoiesis , embryonic stem cell , medicine , genetics , gene , surgery
Numerous efforts have been attempted to regenerate T cells in culture dish from pluripotent stem cells (PSCs). However, in vitro generated T cells exhibited extremely low activity and compromised immunocompetency in vivo. Here, we describe a two-step protocol for regenerating functional T cells using an inducible Runx1-Hoxa9 -PSC (iR9-PSCs) line. The procedure mainly includes generation of induced hematopoietic progenitor cells (iHPCs) in vitro, transplantation, and development of functional induced T cells (iT) in vivo via transplantation. The entire induction process in vitro requires 21 days before iHPCs transplantation. The development of mature T cells in vivo takes 4 to 6 weeks post-transplantation. We provide a simple and reproducible approach for functional T cell regeneration from iR9-PSCs for research purpose.