Open Accessα1,3-Galactosyltransferase-Gene Knockout in Cattle using a Single Targeting Vector with loxP Sequences and Cre-Expressing Adenovirus
Author(s) -
Yutaka Sendai,
Tokihiko Sawada,
M. Urakawa,
Yoichi Shinkai,
Keiichi Kubota,
Hiroyuki Hoshi,
Yoshito Aoyagi
Publication year - 2006
Publication title -
transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.45
H-Index - 204
eISSN - 1534-6080
pISSN - 0041-1337
DOI - 10.1097/01.tp.0000190422.66657.f1
Subject(s) - gene knockout , cre recombinase , gene targeting , gene , biology , genetics , knockout mouse , genetic enhancement , transgene , genetically modified mouse
Gene targeting in large animals has the potential to be useful in medicine as well as in agriculture. Previously, we reported the first successful targeting of the bovine alpha1,3-galactosyltransferase (alpha1,3GT) gene and establishment of a heterozygous knockout cell line. In this report, we generated both heterozygous and homozygous knockout bovine cell lines, and alpha1,3GT-gene knockout cattle.