Open AccessEndothelial Cells Derived from Pigs Lacking Gal??(1,3)Gal: No Reduction of Human Leukocyte Adhesion and Natural Killer Cell Cytotoxicity
Author(s) -
Bettina C. Baumann,
Mårten K J Schneider,
Benjamin G. Lilienfeld,
Maria Antsiferova,
Daniel M. Rhyner,
Robert J. Hawley,
Jörg Dieter Seebach
Publication year - 2005
Publication title -
transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.45
H-Index - 204
eISSN - 1534-6080
pISSN - 0041-1337
DOI - 10.1097/01.tp.0000157231.11083.7c
Subject(s) - xenotransplantation , cytotoxicity , peripheral blood mononuclear cell , immunology , tumor necrosis factor alpha , cell adhesion , microbiology and biotechnology , adhesion , biology , chemistry , transplantation , in vitro , medicine , biochemistry , organic chemistry , surgery
The expression of galactose-alpha(1,3)galactose (Gal) on porcine cells represents a major barrier to xenotransplantation. The generation of Gal-/- pigs to overcome this barrier redirected the focus of research to other rejection mechanisms, including cellular immunity. The present in vitro study investigated (1) the adhesive interactions between human leukocyte subsets and primary endothelial cells derived from inbred Gal-/- and Gal+/+ pigs, and (2) the susceptibility of such Gal-/- porcine endothelial cells to human natural killer (NK) cell cytotoxicity.