Open AccessSuccessful Pancreas Preservation by a Perfluorocarbon-Based One-Layer Method For Subsequent Pig Islet Isolation
Author(s) -
Daniel Brandhorst,
Marcus Iken,
Mathias D. Brendel,
Reinhard G. Bretzel,
Heide Brandhorst
Publication year - 2005
Publication title -
transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.45
H-Index - 204
eISSN - 1534-6080
pISSN - 0041-1337
DOI - 10.1097/01.tp.0000151765.96118.1b
Subject(s) - islet , transplantation , collagenase , viaspan , trypan blue , cold storage , pancreas , chemistry , medicine , andrology , endocrinology , in vitro , biology , diabetes mellitus , biochemistry , enzyme , horticulture
The oxygenation of human pancreas by the two-layer method (TLM) during cold storage was recently established for clinical islet transplantation. Simplification of TLM would facilitate the application of perfluorocarbon (PFC) as a regularly used preservation solution for subsequent islet transplantation. The present study examined whether PFC can be used in a one-layer method (OLM) for long-term pancreas preservation before isolation of adult pig islets.