Open AccessGROWTH FACTORS IMPROVE THE IN VIVO MIGRATION OF HUMAN SKELETAL MYOBLASTS BY MODULATING THEIR ENDOGENOUS PROTEOLYTIC ACTIVITY
Author(s) -
Jean-François Lafrenière,
Philippe Mills,
Jacques P. Tremblay,
Elmostafa El Fahime
Publication year - 2004
Publication title -
transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.45
H-Index - 204
eISSN - 1534-6080
pISSN - 0041-1337
DOI - 10.1097/01.tp.0000131175.60047.eb
Subject(s) - basic fibroblast growth factor , in vivo , myocyte , extracellular matrix , growth factor , proteolytic enzymes , microbiology and biotechnology , matrix metalloproteinase , transplantation , cell migration , biology , c2c12 , in vitro , cell growth , medicine , biochemistry , myogenesis , enzyme , receptor
A main technological problem related to the clinical application of myoblast transplantation is the poor migration of transplanted cells. In this study, we investigated a new physiologic approach that consists of coinjecting motogenic factors insulin growth factor (IGF)-1 or basic fibroblast growth factor (bFGF) to enhance the migration of human skeletal myoblasts. Among the different ways by which those factors can induce the cell migration processes, we investigated their capacity to enhance cell endogenous proteolytic activity that will help transplanted cells to migrate through the extracellular matrix.