Open AccessPB2388 A STUDY TO COMPARE HPC DETERMINED ON THE SYSMEX XN‐9000 WITH FLOWCYTOMETRIC CD34 COUNT IN PERIPHERAL BLOOD AND IN THE HARVESTED PERIPHERAL BLOOD STEM CELL GRAFT FROM AUTOLOGOUS AND ALLOGENIC DONORS
Author(s) -
Mishra S.,
Kulkarni U.,
Mathews N.,
V R.,
Nair S. C.,
George B.,
Mammen D. J.
Publication year - 2019
Publication title -
hemasphere
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 11
ISSN - 2572-9241
DOI - 10.1097/01.hs9.0000568016.49711.b2
Subject(s) - apheresis , cd34 , medicine , stem cell , blood cell , haematopoiesis , peripheral blood mononuclear cell , bone marrow , transplantation , progenitor cell , immunology , surgery , platelet , biology , biochemistry , in vitro , genetics
Background: Hematopoietic stem cell transplantation is the current standard of curative care for all hemato‐lymphoid malignancies as well as of many non‐malignant conditions like marrow failure syndromes, inborn errors of metabolism and immune‐deficiency syndromes. A successful bone marrow transplant requires a minimum of 2‐3 × 10^6 cells/kg patient body weight of CD 34+ cells in the product being transfused. CD34+ cell count in the peripheral blood has the best predictive value for the stem cell yield in the apheresis bag. The major disadvantages of this are the turn‐around time and the cost, besides requiring specialized flow cytometric analysis equipment and trained operators. HPC (Hemtopoietic Progenitor Cells) is a parameter which is measured by automated platforms of Sysmex XN series. Aims: This study aimed to find the reliability of HPC determined on the Sysmex XN‐9000 in comparison with flowcytometric CD34 count in peripheral blood and in the harvested peripheral blood stem cell graft from autologous and allogenic donors. Methods: 102 autologous and allogeneic donors were enrolled in our study after due consent. TLC (Total Leukocyte Count), MNC (Mononuclear cell count), HPC & CD34 assay were done in both the peripheral blood prior to apheresis and the harvest product post‐apheresis. Sysmex‐XN 9000 was used for TLC, MNC and HPC assay. Slides were stained by automated slider stainer and evaluated by the investigator. CD34 Assay was done in flowcytometer using ISH‐AGE single platform protocol. Statistical analysis was done using SPSS 16.0. Results: 67 allogenic and 35 autologous donors were enrolled. 45% were females and 55% males. Correlation between HPC and CD34 was found to be. 887 with p value <0.01 in peripheral blood and. 847 with p value <0.01 in the harvest product. On the other hand TLC had a correlation of. 424 and. 520 in peripheral blood and harvest respectively. MNC had a poor correlation with poor statistical significance. Summary/Conclusion: So, we conclude that HPC is, cheaper and robust method, which is comparable to CD34 in predicting harvest product's adequacy.