Open AccessDifferential Interplay Between Protein Kinase C and Rho-kinase in the Endothelin-1-induced and Pressure-induced Contractions of Rat Posterior Cerebral Artery
Author(s) -
Yoshiaki Okamoto,
Toshihide Kashihara,
Atsushi ogaki,
Kazuo Obara,
Koichi Nakayama
Publication year - 2004
Publication title -
journal of cardiovascular pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.762
H-Index - 100
eISSN - 1533-4023
pISSN - 0160-2446
DOI - 10.1097/01.fjc.0000166281.62915.2c
Subject(s) - protein kinase c , contraction (grammar) , chelerythrine , endothelin 1 , rho associated protein kinase , cytosol , endothelins , medicine , muscle contraction , endothelin receptor , protein kinase a , kinase , endocrinology , chemistry , anatomy , biology , microbiology and biotechnology , biochemistry , receptor , enzyme
To clarify the involvement of protein kinase C and Rho-kinase in the contractile activation of cerebral artery in response to endothelin-1 and pressurization, rat posterior cerebral artery (outer diameter, 100-200 microm) was mounted in arteriograph, and the changes in cytosolic Ca2+ and vessel diameter were measured by video-microscopy in connection with an Argus 50 system. Endothelin-1 (10 nM) induced a tonic contraction with a slight increase in cytosolic Ca2+, which was mostly dependent on protein kinase C (chelerythrine sensitive). Intraluminal pressurization (60 mmHg) also produced contraction with a low cytosolic Ca2+, which was myogenic in nature and dependent on both protein kinase C and Rho-kinase (Y-27632 sensitive). The results suggest differential interplay between protein kinase C and Rho-kinase in the endothelin-1-induced and pressure-induced tonic phase of contractions in the rat posterior cerebral artery.