Open AccessGenotyping Patients With Recent Blood Transfusions
Author(s) -
Michelle N. Gong,
Sheng Yang,
Wei Zhou,
B. Taylor Thompson,
Li Lian Xu,
David C. Christiani
Publication year - 2003
Publication title -
epidemiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.901
H-Index - 173
eISSN - 1531-5487
pISSN - 1044-3983
DOI - 10.1097/01.ede.0000091680.23304.21
Subject(s) - genotyping , medicine , blood transfusion , genotype , genetics , biology , gene
Many studies have used polymerase chain reaction amplification (PCR) to genotype for common polymorphisms in intensive-care units (ICUs) where blood transfusions are common. Evidence that donor leukocytes in transfused blood can be detected by PCR of the recipient blood suggests that this minor population of donor white cells (microchimerism) can interfere with genotyping of allelic polymorphisms in critically ill transfused patients. To investigate this possibility, we assayed DNA extracted from the blood and buccal cells of ICU patients for 2 common polymorphisms in the TNF-beta gene and the surfactant protein-B (SP-B) gene.