Premium
Methodological Characterization of the 2‐Keto [1‐ 13 C]isocaproate Breath Test to Measure in Vivo Human Mitochondrial Function: Application in Alcoholic Liver Disease Assessment
Author(s) -
Parra Dolores,
González Alvaro,
GarcíaVillarreal Luis,
Martínez J. Alfredo
Publication year - 2003
Publication title -
alcoholism: clinical and experimental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 153
eISSN - 1530-0277
pISSN - 0145-6008
DOI - 10.1097/01.alc.0000081623.25175.7e
Subject(s) - breath test , in vivo , alcoholic liver disease , medicine , gastroenterology , endocrinology , nuclear medicine , chemistry , pathology , biology , cirrhosis , microbiology and biotechnology , helicobacter pylori
Background: The 2‐keto[1‐ 13 C]isocaproate oxidation measurement has been shown as a helpful tool in the in vivo assessment of liver mitochondrial function. Methods: The aim of this work was to study the variability of the 2‐keto[1‐ 13 C]isocaproate breath test in 24 healthy controls (8 men and 16 women) and to evaluate its clinical usefulness in 20 patients (14 men and 6 women) with liver disease (7 men with history of alcoholism). Breath test was performed by measuring 13 CO 2 enrichment in breath before and after the oral administration of the tracer and by using isotope ratio mass spectrometry. Results: The intrasubject and intersubject variability of the percentage of tracer oxidized were 8 and 14%, respectively. The 2‐keto[1‐ 13 C]isocaproate oxidation in women was faster ( p = 0.004) and tended to be higher ( p = 0.050) than in men. The percentage of oxidized tracer was lower in those patients with alcoholic liver disease than in healthy volunteers ( p = 0.001) and in nonalcoholic patients ( p = 0.003). Conclusions: The percentage of tracer oxidized appears as a convenient parameter to detect impairment in liver mitochondrial oxidation related to alcoholism by the 2‐keto[1‐ 13 C]isocaproate breath test, establishing different cutoff values depending on gender.