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The Production of Tumor Necrosis Factor‐α by Macrophages in Rats With Acute Alcohol Loading
Author(s) -
Kitazawa Toshiyuki,
Nakatani Yoshihiro,
Fujimoto Masao,
Tamura Nobuhiro,
Uemura Masahito,
Fukui Hiroshi
Publication year - 2003
Publication title -
alcoholism: clinical and experimental research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.267
H-Index - 153
eISSN - 1530-0277
pISSN - 0145-6008
DOI - 10.1097/01.alc.0000078611.55696.f0
Subject(s) - tumor necrosis factor alpha , lipopolysaccharide , proinflammatory cytokine , spleen , macrophage , medicine , kupffer cell , alcoholic liver disease , immunology , necrosis , endocrinology , inflammation , pathology , chemistry , in vitro , biochemistry , cirrhosis
Background: It is suggested that endotoxin, proinflammatory cytokines, and lipopolysaccharide‐binding protein (LBP) play an important role in the development of alcoholic liver disease. Our previous study showed that splenic macrophages were important for endotoxin uptake and excessive production of tumor necrosis factor (TNF) in rats given large amounts of alcohol. To study the pathophysiological roles of macrophages in alcoholic liver diseases, we examined the production of TNF‐α by rat Kupffer cells, splenic macrophages, and alveolar macrophages with acute alcohol loading in the presence or absence of LBP. Methods: Kupffer cells, splenic macrophages, and alveolar macrophages were isolated from male Wistar rats given 5 mg/g body weight of ethanol intraperitoneally after an hour. The production of TNF‐α by these cells incubated with endotoxin 100 ng/ml in the presence or absence of LBP (1% rat serum) was determined. Results: Acute alcohol loading did not affect the production of TNF‐α by Kupffer cells. With acute alcohol loading, splenic macrophages tended to produce more TNF‐α. Alveolar macrophages produced more TNF‐α than Kupffer cells, and although the production of TNF‐α by alveolar macrophages tended to be suppressed by acute alcohol loading, the production of TNF‐α by alveolar macrophages still remained high in the presence of rat serum. Conclusions: Splenic macrophages and alveolar macrophages may be related to excessive production of TNF‐α in acute alcoholics with endotoxemia.