Open AccessPhage-Displayed Mimotopes Recognizing a Biologically Active Anti–HIV-1 gp120 Murine Monoclonal Antibody
Author(s) -
Víctor Raúl Gómez-Román,
Chuanhai Cao,
Yun Bai,
Hugo Santamaría,
Gonzalo Acero,
Karen Manoutcharian,
David B. Weiner,
Kenneth E. Ugen,
Goar Gevorkian
Publication year - 2002
Publication title -
journal of acquired immune deficiency syndromes
Language(s) - Uncategorized
Resource type - Journals
eISSN - 1944-7884
pISSN - 1525-4135
DOI - 10.1097/00126334-200210010-00004
Subject(s) - epitope , monoclonal antibody , antibody dependent cell mediated cytotoxicity , mimotope , antibody , biology , virology , phage display , antigen , recombinant dna , microbiology and biotechnology , immunology , biochemistry , gene
Antibody-dependent cellular cytotoxicity (ADCC) is a host defense mechanism in which Fc receptor-bearing effector cells in combination with antigen-specific antibodies recognize and kill antigen-expressing target cells. The authors previously described a murine monoclonal antibody (MAb-ID6) that mediated ADCC activity against HIV-infected cells. It was demonstrated that the specificity of MAb-ID6 maps to the first 204 amino acids of gp120; however, the exact epitope was not identified. In the present work, by screening phage display libraries with MAb-ID6, the authors have mapped the corresponding epitope to amino acids 86-100 (HIV-1 gp120 sequence). This epitope lies within the C1 region of gp120 and is highly conserved among all subtypes and circulating recombinant forms of HIV-1. Thus, these phage mimotopes of C1 may serve as components of a vaccine for the induction of gp120-specific antibodies mimicking MAb-ID6.