Open AccessDendritic Cells Transduced With HIV Nef Express Normal Levels of HLA-A and HLA-B Class I Molecules
Author(s) -
Lorraine A. Cramer,
Jeffrey A. Frelinger
Publication year - 2001
Publication title -
journal of acquired immune deficiency syndromes
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.162
H-Index - 157
eISSN - 1944-7884
pISSN - 1525-4135
DOI - 10.1097/00126334-200108150-00001
Subject(s) - human leukocyte antigen , cytolysis , downregulation and upregulation , biology , major histocompatibility complex , antigen , virology , immunology , antigen presenting cell , transporter associated with antigen processing , cytotoxic t cell , mhc class i , microbiology and biotechnology , t cell , immune system , in vitro , gene , genetics
HIV Nef protein is important for viral pathogenesis and disease progression. Nef downregulates CD4 and major histocompatibility antigens on the surface of HIV-infected T cells. HIV also infects dendritic cells. We wanted to determine if Nef had a similar function in professional antigen-presenting cells, where downregulation of Class I could have important effects on the initiation of HIV specific cytolytic T cell responses. We infected human dendritic cells with adenovirus expressing Nef. In contrast to T cells and Hela cells, HLA-A and HLA-B molecules are not downregulated nor are other class I molecules increased. We show that, in dendritic cells, HIV Nef has little effect on CD4 or Class I expression.