Open AccessAnnexin 1 Is Secreted in situ during ulcerative colitis in humans
Author(s) -
Vergnolle Nathalie,
Pagès Philippe,
Guimbaud Rosine,
Chaussade Stanislas,
Buéno Lionel,
Escourrou Jean,
Coméra Christine
Publication year - 2004
Publication title -
inflammatory bowel diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.932
H-Index - 146
eISSN - 1536-4844
pISSN - 1078-0998
DOI - 10.1097/00054725-200409000-00013
Subject(s) - annexin a1 , ulcerative colitis , annexin , annexin a2 , inflammatory bowel disease , medicine , secretion , inflammation , colitis , gastroenterology , annexin a5 , extracellular , pathology , disease , chemistry , staining , biochemistry
Although annexin l exerts extracellular anti‐inflammatory properties, little is known about its release in inflammatory diseases. Here, we characterized annexin 1 secretion in ulcerative colitis (UC) patients. Annexin 1 was detected by immunoblotting, in tissue homogenates and supernatants of colonic biopsies incubated in culture media, and in luminal colonic perfusates of UC patients. Annexin 1 was released by inflamed colonic biopsies from patients having severe UC but not by biopsies from healthy colon of the same patient or by biopsies from non‐UC patients or from patients with slight or moderate UC. Annexin 1 was detected in luminal colonic perfusates of patients having moderate or slight UC but not in perfusates from control patients. The level of annexin 1 expression and secretion was unrelated to long‐term glucocorticoid treatment, but annexin 1 secretion in perfusates was induced, in some patients, by short‐term glucocorticoid exposure. These results show that annexin 1 is secreted endogenously in the colon of patients with UC. This secretion, which occurs both in vitro and in vivo, depends on the severity of inflammation. Given the anti‐inflammatory effects of annexin 1, this protein may serve to down‐regulate the inflammatory response in the course of inflammatory bowel disease.