Open AccessALTERATIONS IN MUCOSAL IMMUNE CELL DISTRIBUTION IN SEPTIC RATS
Author(s) -
Johanna Österberg,
Cecilia Johnsson,
G. Gannedahl,
Annemarie Westlund,
Ulf Haglund
Publication year - 1997
Publication title -
shock
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.095
H-Index - 117
eISSN - 1540-0514
pISSN - 1073-2322
DOI - 10.1097/00024382-199703000-00005
Subject(s) - immune system , distribution (mathematics) , immunology , cell , medicine , microbiology and biotechnology , biology , mathematical analysis , mathematics , genetics
A reduced cell-mediated immunity in the intestinal mucosa might promote gut barrier failure and bacterial translocation in shock. This study was performed to investigate changes from the normal distribution of cellular components of the immune system within the intestine during sepsis. Intra-abdominal sepsis was induced by cecal ligation and puncture. After 24 h, the animals were killed and specimens were taken from the small bowel. Immunohistochemical stainings were performed on frozen sections using monoclonal antibodies reactive with MHC class II positive cells (OX6), the alpha/beta antigen receptor on T lymphocytes (R73), CD4+ T lymphocytes (W3/25), CD8+ T lymphocytes (OX8), and macrophages (ED1). There was a significant reduction in the number of pan T lymphocytes as well as both CD4+ and CD8+ subsets in the mucosa of the septic rats as compared with sham operated rats (p < .01). In contrast, the populations of MHC class II positive cells and macrophages increased in sepsis (p < .01). We conclude that 24 h after the induction of peritonitis, there is an altered pattern of immunocompetent cells within the intestinal mucosa.