Open AccessTargeted cloning of cytotoxic T cells specific for minor histocompatibility antigens restricted by HLA class I molecules of interest1
Author(s) -
Yoshiki Akatsuka,
Eisei Kondo,
Hirohumi Taji,
Yasuo Morishima,
Makoto Yazaki,
Yuichi Obata,
Yoshihisa Kodera,
Stanley R. Riddell,
Toshitada Takahashi
Publication year - 2002
Publication title -
transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.45
H-Index - 204
eISSN - 1534-6080
pISSN - 0041-1337
DOI - 10.1097/00007890-200212270-00023
Subject(s) - elispot , human leukocyte antigen , cytotoxic t cell , minor histocompatibility antigen , cd8 , biology , major histocompatibility complex , t cell , antigen , microbiology and biotechnology , transplantation , immunology , immune system , genetics , in vitro , medicine , surgery
T cells specific for minor histocompatibility antigens (mHAgs) play a major role in both graft-versus-host disease and the graft-versus-tumor effect after HLA-identical bone marrow transplantation (BMT). However, characterization of individual T-cell responses to mHAgs is difficult and has generally involved extensive screening of T-cell clones isolated from bulk T-cell cultures generated from BMT recipients. In this report, we describe a new approach that permits both direct visualization of CD8 T-cell responses to mHAgs and cloning of T cells reacting with mHAgs presented by individual HLA alleles of interest.