Open AccessRhesus monocyte-derived dendritic cells modified to over-express TGF-??1 exhibit potent veto activity1
Author(s) -
Clement Asiedu,
Shengli Dong,
Alexander Pereboev,
Weila Wang,
Jesús Ródenas Navarro,
David T. Curiel,
Judith M. Thomas
Publication year - 2002
Publication title -
transplantation
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.45
H-Index - 204
eISSN - 1534-6080
pISSN - 0041-1337
DOI - 10.1097/00007890-200209150-00008
Subject(s) - transduction (biophysics) , cytotoxic t cell , cd40 , microbiology and biotechnology , biology , chemistry , antigen , dendritic cell , immunology , in vitro , biochemistry
The tolerogenic activity of allogeneic bone marrow cells (BMCs) associates with functional inactivation of alloreactive T cells and has been attributed to a veto effect. Studies in mice and rhesus monkeys indicated that the CD8alpha molecule expressed on a subpopulation of allogeneic BMCs is necessary to induce signal transduction within the BMCs to increase veto effector molecules such as transforming growth factor (TGF)-beta1. In vitro activation of alloreactive cytotoxic T-lymphocyte precursor enhances their susceptibility to veto-mediated functional inactivation by specific alloantigen-bearing BMCs. Accordingly, we examined a hypothesis that mature rhesus monkey (Rh) monocyte-derived dendritic cells (MDDCs) modified by gene transfer to over-express active TGF-beta1 might mediate veto activity without the need to express CD8alpha.