Open AccessSERINE-PROTEASE INHIBITION DURING ISLET ISOLATION INCREASES ISLET YIELD FROM HUMAN PANCREASES WITH PROLONGED ISCHEMIA1
Author(s) -
Lakey,
Helms Lm,
Tatsuya Kin,
Korbutt Gs,
Rajotte Rv,
Shapiro Am,
Warnock Gl
Publication year - 2001
Publication title -
transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.45
H-Index - 204
eISSN - 1534-6080
pISSN - 0041-1337
DOI - 10.1097/00007890-200108270-00003
Subject(s) - islet , collagenase , proteases , endocrinology , medicine , serine protease , pancreas , biology , pancreatic islets , microbial collagenase , ficoll , protease , digestion (alchemy) , enzyme , insulin , chemistry , biochemistry , in vitro , chromatography , peripheral blood mononuclear cell
Islet isolation from the pancreatic tissue matrix remains highly variable. Recent evidence suggests that intrinsic human pancreatic proteases, including trypsin, may inhibit effective collagenase enzymatic activity during islet isolation, thereby impairing the isolation success. In this study we have hypothesized that serine protease inhibition applied during pancreatic digestion, could improve yield and/or functional viability of islets isolated from human pancreases.