Open AccessMECHANISM AND PREVENTION OF COLD STORAGE-INDUCED HUMAN RENAL TUBULAR CELL INJURY12
Author(s) -
Abdulla K. Salahudeen,
Hong Huang,
Prakash Patel,
John K. Jenkins
Publication year - 2000
Publication title -
transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.45
H-Index - 204
eISSN - 1534-6080
pISSN - 0041-1337
DOI - 10.1097/00007890-200011270-00005
Subject(s) - cold storage , antioxidant , chemistry , superoxide dismutase , superoxide , biochemistry , glutathione peroxidase , glutathione , biology , enzyme , horticulture
The recent observation that cold storage of kidneys and tubular cells causes marked increase in free radical-catalyzed F2-isoprostanes suggests that radicals might be formed during cold storage. As cold temperature is associated with reduced metabolic and enzymic activity, the notion that cold temperature causes free radical production appeared less tenable. The objective was, therefore, to seek direct evidence for the free radical production during the cold storage of human renal tubular cells, and to define the roles of extrinsic and intrinsic antioxidants in cold-induced cell injury.