Open AccessRETROVIRAL DELIVERY OF VIRAL INTERLEUKIN-10 INTO MYELOID DENDRITIC CELLS MARKEDLY INHIBITS THEIR ALLOSTIMULATORY ACTIVITY AND PROMOTES THE INDUCTION OF T-CELL HYPORESPONSIVENESS1,2
Author(s) -
Takuya Takayama,
Yasuhiko Nishioka,
Lina Lü,
Michael T. Lotze,
Hideaki Tahara,
Angus W. Thomson
Publication year - 1998
Publication title -
transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.45
H-Index - 204
eISSN - 1534-6080
pISSN - 0041-1337
DOI - 10.1097/00007890-199812270-00001
Subject(s) - dendritic cell , biology , t cell , bone marrow , immune system , cytotoxic t cell , microbiology and biotechnology , viral vector , flow cytometry , transgene , cytokine , immunology , gene , in vitro , recombinant dna , biochemistry
Dendritic cells (DC) play critical roles in the initiation and modulation of immune responses and may determine the balance between tolerance and immunity. Viral interleukin-10 (vIL-10), encoded by the Epstein-Barr virus, is highly homologous to the "immunosuppressive" cytokine, mammalian IL-10. It impairs antigen-presenting cell function but lacks certain immunostimulatory properties of mammalian IL-10. We accomplished the following: (1) evaluated the effects of vIL-10 protein on DC phenotype and function, (2) transduced mouse bone marrow-derived DC to express vIL-10, and (3) assessed the impact of transgene expression on DC allostimulatory activity.