Open AccessSTANDARDIZATION OF PROCEDURE FOR EFFICIENT EX VIVO GENE TRANSFER INTO PORCINE PANCREATIC ISLETS WITH CATIONIC LIPOSOMES1
Author(s) -
Pierre Yves Benhamou,
Christine Moriscot,
Philippe Prevost,
E. Rolland,
Serge Halimi,
Jadwiga Chroboczek
Publication year - 1997
Publication title -
transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.45
H-Index - 204
eISSN - 1534-6080
pISSN - 0041-1337
DOI - 10.1097/00007890-199706270-00016
Subject(s) - islet , transplantation , biology , transfection , microbiology and biotechnology , genetic enhancement , pancreatic islets , gene expression , viral vector , reporter gene , collagenase , cationic liposome , adeno associated virus , gene , vector (molecular biology) , medicine , endocrinology , insulin , biochemistry , enzyme , recombinant dna
New strategies to improve the outcome of encapsulated porcine islet transplantation may involve the transfer of gene sequences affecting islet viability. While adenoviral vectors appear as the most efficient gene transfer system so far established for islets, non-viral-based vectors are most likely to fulfill microbiological safety criteria and be retained in the clinical setting. Our aim was to standardize the procedures of gene transfer into adult porcine islets using cationic liposome DOTAP.