Open AccessDONOR ORIGIN OF A POSTTRANSPLANT LIVER ALLOGRAFT MALIGNANCY IDENTIFIED BY FLUORESCENCE IN SITU HYBRIDIZATION FOR THE Y CHROMOSOME AND DNA GENOTYPING
Author(s) -
John A. Donovan,
Fred A. Simmons,
Karl T. Esrason,
Mehdi Jamehdor,
Ronald W. Busuttil,
Jessicca M. Novak,
Wayne W. Grody
Publication year - 1997
Publication title -
transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.45
H-Index - 204
eISSN - 1534-6080
pISSN - 0041-1337
DOI - 10.1097/00007890-199701150-00015
Subject(s) - malignancy , fluorescence in situ hybridization , liver transplantation , genotyping , transplantation , pathology , y chromosome , in situ hybridization , biology , chromosome , biopsy , medicine , genetics , gene , genotype , gene expression
Posttransplantation malignancy in the allograft is a rare complication of orthotopic liver transplantation. In the described case, an abnormal T-tube cholangiogram, performed 6 months after orthotopic liver transplantation between a male donor and a female recipient, prompted needle liver biopsy. A moderately differentiated adenocarcinoma was found. Fluorescence in situ hybridization for the Y chromosome indicated male origin of malignancy. Donor-related disease was confirmed by comparative DNA analysis of genomic sequences from the donor liver, associated tumor, and recipient peripheral blood. Results of these investigations qualified the recipient for a second liver transplant.