Open AccessSTIMULATION OF CD4+ T LYMPHOCYTES BY ALLOGENEIC MHC PEPTIDES PRESENTED ON AUTOLOGOUS ANTIGEN-PRESENTING CELLS
Author(s) -
Kay E. Parker,
Rosemarie Dalchau,
Valerie J. Fowler,
Carol A. Priestley,
Clare A. Carter,
John W. Fabre
Publication year - 1992
Publication title -
transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.45
H-Index - 204
eISSN - 1534-6080
pISSN - 0041-1337
DOI - 10.1097/00007890-199204000-00038
Subject(s) - cd8 , major histocompatibility complex , mhc restriction , antigen , mhc class i , peptide , cytotoxic t cell , immunology , t cell , biology , mhc class ii , antigen presentation , immune system , in vitro , microbiology and biotechnology , chemistry , biochemistry
A preliminary analysis of the alloantibody response to free, unconjugated class I and class II MHC peptides in several rat and mouse strains was performed, to screen for an effective interaction between the allogeneic MHC peptides and recipient MHC molecules. The PVG rat strain was noted to produce very strong, MHC-restricted, primary and secondary responses to a synthetic peptide derived from the alpha helical region of the alpha 2 domain of an RT1.C/E class I MHC molecule of the DA strain. In vitro proliferation studies demonstrated that CD4+ but not CD8+ T cells of the PVG strain responded in a recipient APC-dependent manner to the peptide, whereas the BN strain (which showed no antibody response to this peptide) gave no T cell proliferation. Immunization of PVG rats with the peptide did not influence the rejection of DA skin allografts. The relevance of these studies to the possible mechanisms of allograft rejection by an indirect pathway are discussed.