Open AccessThe Depletion of T Cell Subsets in Vitro and in Vivo
Author(s) -
Stephen Cobbold,
Graham D. Martin,
Herman Waldmann
Publication year - 1986
Publication title -
transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.45
H-Index - 204
eISSN - 1534-6080
pISSN - 0041-1337
DOI - 10.1097/00007890-198609000-00003
Subject(s) - monoclonal antibody , bone marrow , immunology , transplantation , histocompatibility , bone marrow transplantation , t cell , antibody , biology , monoclonal , major histocompatibility complex , medicine , antigen , immune system , human leukocyte antigen
One of the major complications of allogeneic bone marrow transplantation is graft-versus-host disease. This can be avoided by removing the mature T cells from the marrow, most conveniently by the use of monoclonal antibodies. However, T cell purging results in an increased tendency for the recipient to reject the donor marrow. We have developed monoclonal antibodies to L3/T4 and Lyt-2 that specifically deplete functional T cell subsets in mice. We demonstrate that such reagents can be used to control both graft-versus-host disease and marrow rejection in mouse models of bone marrow transplantation across one-haplotype or two-haplotype major histocompatibility differences. Such strategies to abrogate host resistance, by administration of anti-T-cell monoclonal antibodies to the recipient, may complement marrow T cell purging for human allogeneic bone marrow transplantation.