Open AccessGAMMA-INTERFERON PRODUCTION CAPACITY AND T LYMPHOCYTE SUBPOPULATION AFTER ALLOGENEIC BONE MARROW TRANSPLANTATION1
Author(s) -
Shinichi Mizuno,
Yasuo Morishima,
Yoshihisa Kodera,
Ryuzo Ohno,
Syozo Yokomaku,
Hiroshi Sao,
Satoshi Yoshikawa
Publication year - 1986
Publication title -
transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.45
H-Index - 204
eISSN - 1534-6080
pISSN - 0041-1337
DOI - 10.1097/00007890-198603000-00006
Subject(s) - immunology , lymphocyte , antigen , bone marrow , t lymphocyte , transferrin receptor , monoclonal antibody , t cell , interferon , population , biology , medicine , antibody , immune system , transferrin , endocrinology , environmental health
T cell phenotypes after bone marrow transplantation (BMT) were investigated using monoclonal antibodies (moAbs) reactive to lymphocyte cell surface antigens. Patients' T cells showed decreased percentages of OKT4, 4A and 9.3-positive T cells, and increased percentages of OKT8, human Ia, and Leu-7-positive T cells. These changes in T cell phenotype persisted for a long period after BMT and had no correlation with the occurrence of graft-versus-host disease (GVHD). No lymphocyte activation antigens such as TIA (Tac) or transferrin receptor (5E9) were detected after BMT. The capacity of the patients' lymphocytes to produce gamma-interferon (IFN-gamma) was measured after incubation of lymphocytes with mitogen. Patients' lymphocytes produced significantly lower levels of IFN-gamma than the normal controls. This failure of IFN-gamma production showed no correlation with stimulation index of mitogen blastogenesis or changes of T cell population. Thus, not only T cell phenotype but also measurement of IFN-gamma production of lymphocyte may be useful in detecting immunological abnormalities in patients who receive BMT.