Open AccessCELL-MEDIATED CYTOTOXICITY PATTERNS OF COLONED CYTOTOXIC T LYMPHOCYTES
Author(s) -
Gerard Groenewegen,
Wim A. Buurman,
C. J. Van Der Linden,
G. M. A. A. Jeunhomme,
G. Kootstra
Publication year - 1985
Publication title -
transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.45
H-Index - 204
eISSN - 1534-6080
pISSN - 0041-1337
DOI - 10.1097/00007890-198506000-00016
Subject(s) - cytotoxic t cell , ctl* , cytotoxicity , lymphoblast , effector , immunology , antigen , lysis , biology , t lymphocyte , lymphocyte , microbiology and biotechnology , cell culture , in vitro , biochemistry , genetics
Knowledge of the antigens recognized during allograft rejection is still incomplete. Cloned cytotoxic T lymphocytes were used to study the distribution of target determinants in the dog. CTL clones were obtained with a limiting dilution technique from effector cells generated in mixed lymphocyte culture. The clones have been tested for cell-mediated cytotoxicity against PHA-stimulated lymphoblasts, monocytes, and arterial and venous endothelial cells. A limited number of patterns of lysis of one, or more than one, of the four different target cells was observed. The nature of the possible target determinants recognized by these CTL-clones is discussed.