Open AccessBONE MARROW REPOPULATION CAPACITY AFTER TRANSPLANTATION OF LYMPHOCYTE-DEPLETED ALLOGENEIC BONE MARROW USING COUNTERFLOW CENTRIFUGATION
Author(s) -
Théo de Witte,
Reinier Raymakers,
A. Plas,
E. Koekman,
H. Wessels,
C. Haanen
Publication year - 1984
Publication title -
transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.45
H-Index - 204
eISSN - 1534-6080
pISSN - 0041-1337
DOI - 10.1097/00007890-198402000-00007
Subject(s) - bone marrow , centrifugation , differential centrifugation , transplantation , progenitor cell , repopulation , immunology , stem cell , andrology , microbiology and biotechnology , medicine , biology , chemistry , chromatography , surgery , haematopoiesis
Bone marrow from six allogeneic HLA-matched and MCL nonreactive siblings was fractionated by means of isopycnic flotation centrifugation and subsequent counterflow centrifugation. The low density fraction (d less than or equal to 1.070 g/ml) obtained by IFC contained 20% of the nucleated cells and more than 90% of the myeloid and erythroid progenitors. The putative stem cell fraction obtained by CC showed a satisfactory recovery (88%) of the CFU-GM and BFU-E and only 3.5% of the original number of T lymphocytes. Bone marrow repopulation capacity was not impaired in comparison with a comparable group of patients. Despite the average high age of this group (29.6 years), only one of the four evaluable patients developed graft-versus-host disease.