Open AccessSUPPRESSION OF MIXED LYMPHOCYTE REACTIVITY BY CELLULAR AND HUMORAL FACTORS IN APLASTIC ANEMIA—BOTH BEFORE AND AFTER BONE MARROW TRANSPLANTATION
Author(s) -
Elizabeth Naparstek,
A. Amar,
Chaim Brautbar
Publication year - 1983
Publication title -
transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.45
H-Index - 204
eISSN - 1534-6080
pISSN - 0041-1337
DOI - 10.1097/00007890-198310000-00003
Subject(s) - aplastic anemia , immunology , lymphocyte , medicine , bone marrow , human leukocyte antigen , mixed lymphocyte reaction , transplantation , antibody , anemia , histocompatibility , bone marrow transplantation , antigen , immune system , t cell
A patient with infectious hepatitis who developed severe aplastic anemia received a bone marrow transplant from her HLA-identical, mixed lymphocyte culture (MLC)-negative sister. It was found that pretreatment of normal lymphocytes with the immunoglobulin fraction of the patient's serum resulted in marked inhibition of their proliferative response to mitogens, as well as their ability to serve as stimulators and responders in MLC. The patient's lymphocytes, unlike those of her HLA-identical sister were unable to stimulate and respond in MLC and markedly suppressed mixed lymphocyte reactivity between two unrelated healthy individuals. Donor-type lymphocytes obtained from the patient after engraftment were also unable to respond or stimulate in MLC. It is suggested that the suppression of lymphocyte responses was mediated by an immunoglobulin present in the patient's serum.