Open AccessEFFECT OF IN VITRO CYCLOSPORIN A TREATMENT ON HUMAN NATURAL AND ANTIBODY-DEPENDENT CELL-MEDIATED CYTOTOXICITY
Author(s) -
Shao-Hsien Chen,
István Làng,
Han Gunn,
Peter M. Lydyard
Publication year - 1983
Publication title -
transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.45
H-Index - 204
eISSN - 1534-6080
pISSN - 0041-1337
DOI - 10.1097/00007890-198302000-00004
Subject(s) - antibody dependent cell mediated cytotoxicity , effector , cytotoxicity , k562 cells , antibody , in vitro , lysis , lymphokine activated killer cell , natural killer cell , immunology , chemistry , secretion , biology , microbiology and biotechnology , pharmacology , interleukin 12 , cytotoxic t cell , biochemistry
Concentrations of 0.1 to 10.0 micrograms of cyclosporin A (Cy A) per ml do not directly inhibit the lysis of 51Cr-labeled K562 target cells by human peripheral blood natural killer (NK) cells nor of antibody-coated chicken red blood cells by antibody-dependent cellular cytotoxicity (ADCC) effector cells. Pretreatment of effector cells with Cy A for 18 hr still failed to affect NK or ADCC effector function. However, induction of NK cell activity by overnight pretreatment of effector cells by polyinosinate-polycytidylate (PolyIC) is inhibited by as little as 1 micrograms of Cy A per ml. The mechanism of inhibition may involve a direct effect of Cy A on "induced" or "activated" NK cells or inhibition of the release of Poly IC-induced interferon from lymphocytes.