Open AccessMACROPHAGE ELECTROPHORETIC MOBILITY TEST AS A SENSITIVE PROBE OF TRANSPLANTATION IMMUNITY IN MICE
Author(s) -
M Malkovský,
Jan Bubenı́k,
Michael Boubelı́k,
P Hausner
Publication year - 1979
Publication title -
transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.45
H-Index - 204
eISSN - 1534-6080
pISSN - 0041-1337
DOI - 10.1097/00007890-197908000-00010
Subject(s) - histocompatibility , transplantation , immunity , macrophage migration inhibitory factor , macrophage , antigen , immunology , cellular immunity , microbiology and biotechnology , strain (injury) , lymph node , major histocompatibility complex , electrophoresis , chemistry , biology , in vitro , immune system , medicine , human leukocyte antigen , biochemistry , anatomy , cytokine
Transplantation immunity was examined in mouse H-2- and non-H-2-disparate strain combinations by the macrophage electrophoretic mobility (MEM) assay. Lymph node cells from the recipients rejecting histoincompatible skin grafts were incubated in the presence of solubilized histocompatibility antigens prepared from spleens of the grafts donors. Product(s) of lymphocyte-antigen interaction in appropriate combinations (sensitized lymphocytes with the sensitizing antigen) significantly diminished the electrophoretic mobility of guinea pig macrophages in all of the histoincompatible strain combinations examined. The data indicate that transplantation immunity in mice can be detected by the MEM assay.